Computer Controlled Microscopy

OPEN SOURCE ARDUINO FOR EXISTING MICROSCOPES...INTRODUCING RetroBOT BY BUNTON

Much has changed in twenty-five years. We now make our own 3D-printed motorized XYZ system called RetroBOT. The controller is Arduino technology (open source) and provides amazing XYZ control for a fraction of the price one would spend for a conventional motorized stage made by subtractive manufacturing (metal removal) with expensive controller cards and proprietary electronic control boxes. These stages are quickly installed on modern upright microscopes. The target market is still pathology and materials science (including geological samples). The big difference today is the much lower price tag and highly efficient software that is useable for digital image stitching and stacking methods. The cameras are all  high resolution digital CMOS with global shutters and USB3 connection to a Windows 10 computer. RetroBOT is the only commercial system on the market that is 3D printed and ready for special applications as well as routine image capture and processing.

ENTER RetroZED FOR Z-STACKING WITH YOUR DSLR CAMERA......
Another new Bunton product is called RetroZED (z-axis extended depth). This modular Z-drive connects to just about any coaxial fine focus drive .... upright or inverted microscopes. Main mission: Automated Z-stacking. Dr. Brian Matsumoto was kind enough to submit the great diatom photomicrographs you see below. The first set are single focal plane views of the top and bottom of the 3D diatom structure. He also shows the girdle profile of the diatom! An Olympus BX 51 microscope with 40x DIC objective was used. The second photomicrograph illustrates the fantastic power of EDF (extended depth of focus). The completed stack is composed of about 40 individual focal planes spaced 1 micron apart. Sub-micron stepping of the fine focus is easily achieved for smaller structures when using RetroZED.

We use the Cognisys Stackshot controller and either Zerene Stacker or Helicon Focus software. It is designed for use with modern consumer DSLR and MILC large format cameras. Some of today's large format cameras have sensors the same size as a frame of 35mm photo film .... 43mm diagonal. Megapixel performance of about 50MP is available at a reasonable price. A Panasonic S1r full frame digital camera was used by Dr. Matsumoto for capturing the images you see here. The basic RetroZed kit (less software and camera) is under $800.00. This includes the controller, power supply, cable, cable Z-drive and stand plus the simple connector to use the system on just about any fine focus knob. Different diameter, interchangeable "gripper cups" are available for a nominal price. No tools needed.  Go to YouTube and search: Matsu Imaging Diatom Video

AUGMENTED REALITY (AR) MICROSCOPY

AUGMENTIQS MODULE FOR HIGH RESOLUTION, WIDE FIELD OF VIEW DIGITAL PHOTOMICROSCOPY AND TELEPATHOLOGY USING YOUR EXISTING INFINITY CORRECTED TUBE LENGTH MICROSCOPE
THE MODULE STARTS AT $7,100.00 (DELIVERED TO YOUR FACILITY) AND ONCE CONNECTED TO YOUR WINDOWS O/S COMPUTER YOU WILL BE ALL SET FOR 5MP (GLOBAL SHUTTER SONY CMOS SENSOR CAMERA) PHOTOMICROSCOPY WITH A FIELD OF VIEW SIGNIFICANTLY LARGER THAN THAT ACHIEVED USING AN EXTERNAL SMALL OR LARGE FORMAT CAMERA ON A CAMERA PORT. THIS IS NOT A WSI IMAGE STITCHING SYSTEM.....SEE OCUS OR MOTIC EASY SCAN FOR THOSE OFFERINGS....OR STAY TUNED FOR THE NEW MANUAL WSI SOFTWARE MODULE TO ENABLE BOTH AUGMENTED REALITY AND WHOLE SLIDE IMAGING.   ONE SYSTEM FOR TELEPATHOLOGY, PHOTOMICROSCOPY, ANNOTATIONS, MEASUREMENTS AND WSI.

AUGMENTIQS OFFERS OPTIONS FOR A SECONDARY CAMERA THAT MONITORS THE OBJECTIVE LENS BEING USED AND SLIDE XY POSITION
AUGMENTIQS OFFERS AN OPTION FOR A GLOBAL SHUTTER CAMERA AND SOFTWARE FOR ARTIFICIAL INTELIGENCE IMAGE CAPTURE TO BE USED FOR BUILDING AI DATASETS.....ALL OPEN SOURCE     VISIT: www.airamatrix.com (INDIA)   "KIKO"....KNOWLEDGE IN....KNOWLEDGE OUT
ANNUAL LICENSE FOR UNLIMITED TELEPATHOLOGY, REPORTING, SMS/EMAIL AND QUANTITATIVE ANALYSIS OPTION
ABOVE UNIT SHOWN WITH A EXTERNAL MINI-CAM FOR MONITORING OBJECTIVE MAGNIFICATION (AND CALIBRATION OF SAME) PLUS XY STAGE POSITION
THE AR MODULE OPTION ($3,500.00) ALLOWS FOR THE OVERLAY IN THE OCULARS
BASIC MODULE ALLOWS FOR DIRECT VIEWING ON YOUR COMPUTER DISPLAYER
IDEAL VIEWING TUBE WILL HAVE THE ERGONOMIC TILT FEATURE FOR COMFORTABLE OCULAR VIEWING WITH THE MODULE IN POSITION
COMPLETE TELEPATHOLOGY SYSTEMS START AT ABOUT $14,000.00
HIGH RESOLUTION OCULAR VIEWING WITH ALL OBJECTIVE LENSES
AUGMENT YOUR MICROSCOPY WITH AUGMENTIQS!

NEW DUAL CAMERA MODULE IS COMING IN 2021 FOR FLUORESCENCE ARM
CONTACT US ABOUT UPGRADING YOUR INFINITY CORRECTED UPRIGHT MICROSCOPE TO A SMART MICROSCOPE
CONVERT YOUR HALOGEN LAMP TO LED FOR CONSTANT COLOR TEMPERATURE AT ALL INTENSITY LEVELS
USERS INCLUDE CDC AND FDA - INCLUDES NEW ALGORITHM FOR KI-67 CELL DETECTION
A GREAT PODCAST (47 MINUTES) BETWEEN GABE SIEGEL (CEO OF AUGMENTIQS IN ISRAEL) AND DR. ALEKSANDRA ZURAW OF www.digitalpathologyconsulting.com IS A VERY USEFUL SESSION THAT DISCUSSES THE HISTORY OF AUGMENTIQS AND THEIR PRODUCTS FOR DIGITAL MICROSCOPY.
LISTEN TO THE PODCASE AND GET IN TOUCH WITH US IF YOU WOULD LIKE MORE DETAILS AND PRICING
INTEREST IN "AI"?...... VISIT: www.mindpeak.ai in HAMBURG, GERMANY
TURBO-CHARGE YOUR EXISTING MICROSCOPE - ARM (AUGMENTED REALITY MICROSCOPY) AND WSI (WHOLE SLIDE IMAGING) ON THE SAME INFINITY CORRECTED UPRIGHT MICROSCOPE - CONTACT US WITH YOUR MAKE AND MODEL MICROSCOPE AND WE WOULD BE HAPPY TO DISCUSS YOUR REQUIREMENTS AND PREPARE A PRICE QUOTE FOR YOUR APPROVAL.


CENTER IMAGE is the SIDC for early adopters and developers wanting to integrate their own software with our hardware
Open Source API and firmware included with each RetroBOT developer kit
PHOTO RIGHT shows our latest IZ controller (Arduino and driver modules) with stepper motor and gripper cup
DEVELOPMENT COMING FOR AUTOMATED STITCHING AND STACKING ROUTINES

PHOTO LEFT: Latest RetroBOT XYZ system with the Augmentiqs AR module.....note the color coded objective ID marker on each lens. The external stage camera monitors these markers so that pre-calibration morphometrics are quickly set via software. Another "smart" marker on the stage allows imaging of the slide label (bar code) as well as accurate XY co-ordinates for field of view location. AI and AR plus high resolution, widefield 20MP photomicroscopy and telepathology features.   OUR SOLUTION: REMOTE ROBOTIC MICROSCOPY!
THE EXPERT CONTROLS THE MICROSCOPE AND MAKES A DIAGNOSIS FROM A REMOTE LOCATION

COMING SOON ....STACK AND STITCH

3D PRINTED COMPONENTS DESIGNED AND MADE IN THE USA - MOTORIZED XYZ COMPUTER CONTROLLED PHOTOMACROGRAPHY AND PHOTOMICROSCOPY


THE CONCEPT IS SIMPLE.    XYZ STEPPER MOTORS CONTROLLED BY SOFTWARE TO PERMIT Z-STACKING AND STITCHING OF IMAGES ALL WITH ONE SYSTEM.   RetroFOT WILL REVOLUTIONIZE DIGITAL PHOTOMACRO/MICRO CAPTURE FOR HIGH RESOLUTION GIGAPIXEL IMAGES THAT CAN BE SAVED AND VIEWED CONVENIENTLY WITH A VARIETY OF CLOUD VIEWERS.
LET US KNOW IF YOU WOULD LIKE TO BE KEPT ADVISED OF PROGRESS.....WE APPRECIATE YOUR INTEREST AND SUGGESTIONS.

RetroFOT IS COMING SOON



GIGAMACRO STITCH AND STACK ROBOTIC CAMERA...MACRO OR MICRO WITH A DSLR CAMERA

GIGAMACRO USA .... the Magnify2 is table top robotic photomacro/micro system for use in the lab. This is the only such system on the market for "stack and stitch". Gigapixel virtual images are created with amazing clarity and depth of focus. Using a modern large format camera (DSLR or MILC) and USA-made robotics, this system combines Z-stacking (Zerene or Helicon) with precision X/Y image stitching. Magnify2 has the sample in a stationary posistion while the camera, optics, flash and auxillary lighting is mounted to a motorized gantry. This is an ideal system for creating virtual images of large or small objects. Museum collection trays to ballistics and electronics .... Magnify2 is ready to handle challenging and repetitive tasks that would be nearly impossible to accomplish manually.
Basic instrument with computer, macro lens, DSLR software and flash starts at $48,000.00.


MAGNIFY 2 IS AVAILABLE FOR DEMO ON BOTH COASTS......LET ME KNOW IF YOU ARE INTERESTED

micro3d@comcast.net - 301-831-3434 in Mount Airy, Maryland
Contact Person: Jim Averill, President
FAX: 301-831-3424

HIGH SPEED WSI SCANNER FOR MULTIPLE SLIDES AND OBJECTIVE LENSES

MOTIC'S NEWEST EASYSCAN PRO 6 HAS TWO APO OBJECTIVES AND WILL HANDLE SIX SLIDES OR MORE....STARTS AT $30,000.00 WITH SOFTWARE AND SUITABLE WINDOWS 0/S COMPUTER. ASK ABOUT EASYSCAN ONE "FS" FOR SINGLE SLIDE FROZEN SECTION WSI SCANNING....$20,000.00.
Visit: www.moticeasyscan.com