BUNTON OFFERS A WIDE SELECTION OF MICROSCOPE ILLUMINATION SOLUTIONS.....LED IS RAPIDLY REPLACING THE INCANDESCENT LIGHT BULB AS WELL AS ARC LAMPS THAT HAVE BEEN USED FOR THE PAST 40 YEARS IN FLUORESCENCE MICROSCOPY
Please feel free to contact us with your exact requirements for lighting. We are specialists in adapting new LED illumination for older, existing legacy microscopes. Constant color temperatures and very even illumination. New products now can provide special illumination that was not possible in the past. Transmitted illumination as well as incident light illumination is now optimized using LED modules.
ANNOUNCING NEW LED ILLUMINATION RETRO-FITS TO REPLACE MERCURY ARC LAMPS ON YOUR EXISTING MICROSCOPE...ONE, TWO, THREE OR FOUR LAMBDA LED ILLUMINATORS
Suggested viewing: "INTRODUCTION TO FLUORESCENCE MICROSCOPY" on YouTube via www.ibiology.org
from Dr. Nico Stuurman for newcomers to fluorescence microscopy
OUR NEWEST LED IS SHOWN BELOW IN PHOTOS #2 AND #3. This 3 spike unit is a direct replacement for HBO mercury arc lamps. The purchase price of the unit is under $3,000.00 ....about the same as purchasing FIFTEEN (15) MERCURY ARC BULBS! Set intensity levels of each LED spike to your liking....try doing that with an arc lamp. Image #1 LEFT) was captured using a 60x water immersion objective with NA=1.2 using this LED module (Brian Matsumoto, photomicroscopist). BOVINE PULMONARY ALVEOLAR CELLS STAINED WITH DAPI (BLUE), FITC (GREEN) AND RHODAMINE (RED). A CONSUMER PANASONIC DSLR G9 WAS USED ALONG WITH COLOR MERGE ON THREE EXPOSURES IN PHOTOSHOP.
20,000 HOUR LIFETIME - NO HOT SPOT - COOL OPERATION - CONTROL VIA YOUR COMPUTER - EACH SPIKE HAS A SHUTTER - ADAPTER INCLUDED
THIS LED IS SIGNIFICANTLY BETTER IN THE 450NM (BLUE) WHEN COMPARED TO MERCURY ARC - IDEAL FOR MICROPLASTIC INVESTIGATIONS - HIGHLY STABLE LIGHT INTENSITY THROUGHOUT THE LED LIFETIME - REDUCED ENERGY COSTS - NO WARM-UP OR COOL-DOWN PERIOD - VARIABLE INTENSITY
LOWER ENERGY COSTS AND GREEN FOOTPRINT TO HELP REDUCE GLOBAL WARMING
EXTERNAL LED FOR WHITE LIGHT OR FLUORESCENCE STUDIES AT LOW MAGNIFICATION .... MADE IN AMERICA
PHOTO #1 BELOW: USA-made Infinity Photo Optical TS-160 with your DSLR or MILC attached for high resolution digital imaging with external LED illuminators. Emitter (barrier) filter is easily interchangeable. Far better results than using inexpensive stereo microscopes with off-axis camera ports. Built-in fine focus and aperture diaphragm make this a perfect solution for Z-STACKING of 3D fluorescing samples.
PHOTO #2 BELOW: This is the versatile ADAPTALUX XENON FLASH HEAD kit from the UK. ORDER DIRECT ON LINE FROM ADAPTALUX AND MENTION BUNTON INSTRUMENT #165 ON THE CHECKOUT PAGE UNDER ORDER NOTES. GREAT VALUE WITH THE POUND NEAR PARITY WITH THE US $$.
NOW SHIPPING FROM THE USA WAREHOUSE FOR FAST DELIVERY AND PRICES THAT INCLUDE SHIPPING AND IMPORT COSTS. PAYPAL AND MOST CREDIT CARDS ARE ACCEPTED.
PHOTO #3 BELOW is a USA-made LED modeling light with a beamsplitter to attach to your compound microscope lamp port. A flash unit for photomicroscopy of aquatic organisms or z-stacking with synch control from the Stackshot controller.....more innovative illumination is coming from BUNTON!
Bunton is soon to launch the new RetroFOT-Z with our RetroFL attachment for incident light fluorescence studies and z-stacking. This unique system features the USA-made Infinity KC VIDEOMAX microscope front that can be used with infinity-corrected macro objectives (IF-Series) as well as any infinity-corrected microscope objective...Infinity Achrovid, Mitutoyo M26 thread or any of the NOZL firm industrial objectives for your specific lighting requirements.
NOTE: Here is our prototype RetroFL with the blue light LED attached. Use with just about any camera including Peltier-cooled models (as shown LEFT). Delivery will begin Q1-2022. Contact us for further details, questions or price quote. The LED you see is made in the USA and sells for about $1,000.00 plus an adapter for your microscope make and model. Older Leitz lamp housings accepted on trade-in.....no mercury sockets or older power supplies needed! Save a few bucks and go LED with a trade-in allowance.
The USA-made LED illuminator you see here is offered with 400, 450, 500 and 550nm LED matrix and collector lens. Intensity can be easily adjusted to your exact requirements with the control knob shown on top of the heat sink metal housing. Filter cubes and LED sources are quickly exchanged as needed for different fluorochromes. Very reasonably priced!
The KC features internal fine focusing and an aperture diaphragm. Long working distance lenses make this system ideal for intravital studies as well as for fluorescence of embryos and larger organisms that would not be suitable for regular compound microscopes. Ideal setup for the imaging of MICROPLASTICS in water samples and seafood. Coaxial fluorescence microscopy with digital imaging and software for measurements, counting and report documentation. This system (RetroFL) provides much better results than a routine stereoscopic microscope with a camera mounted on one of the two optical channels. Micro or macro front optics...interchangeable filter cubes and LED modules.
Filter cubes are quickly exchanged with the removal of screws. 25mm filter dimensions provide a very wide field of illumination and the LED matrix is quite homogeneous over a 20-30mm field depending on placement of the lamp in the illumination port. A collector lens is built into the LED ILLUMINATOR. Contact BUNTON for a price quote on the KC-RetroFL with LED illumination.
NEW RetroFL INCIDENT LIGHT FLUORESCENCE MODULE WITH LED ILLUMINATION (PHOTO LEFT)
We have been building specialized microscopy systems for many years now. The RetroFL 3D-printed module (RIGHT) is our third generation prototype. It is designed for functionality, easy filter module and LED interchange and light weight. We use the special enlarged cubes from Chroma and any filter sets in their catalog may be used with RetroFL. Note the exciter filter of a GFP "cube" inside the RetroFL. This is the interchange illumination port. The black metal adapter on top receives the Infinity Photo Optical KC microscope. The front lens is then mounted on the bottom of the RetroFL module in another metal threaded mount. The third photo at the end of this page shows our two earlier versions of RetroFL. The easily interchangeable fitler cubes and LED modules make this a very unique tool.
We presently offer LED single spectra illuminators for ROYAL BLUE and GREEN excitation. This is a perfect modular solution for the study of NILE RED stained MICROPLASTICS found in our waters and fisheries. NILE RED is excited with the 450-480nm ROYAL BLUE. Such lighting and filter cubes are also useable for GFP and FITC. The INFINITY KC microscope is then attached along with your camera of choice. Software for Z-STACKING or IMAGE ANALYSIS can then be used to meet your exact requirements. MICROPLASTIC MICROSCOPY involves plastic particles 5MM OR LESS. The KC microscope can be easily converted from MACRO to MICRO for single digit MICRON SIZED particle imaging and identification. NEW FOR 2023....MODULAR LED FOR NUV EXCITATION IN THE 370NM RANGE. CONTACT US FOR A CUSTOM-MADE RetroFL WITH KC-VIDEOSCOPE TO MEET YOUR RESEARCH REQUIREMENTS. USE YOUR EXISTING INFINITY-CORRECTED OBJECTIVES INCLUDING MITUTOYO AND SPECIAL NOZL OPTICS FOR LONG WORKING DISTANCE AND HIGH N.A.
WE WILL SOON HAVE A MULTI-SPECTRA LED ILLUMINATOR WITH LIQUID LIGHT GUIDE AVAILABLE FOR USE WITH RetroFL. Affordable and perfect for intravital microscope configurations where a heavy lamp housing connected to the main microscope would be unsatisfactory. Stay tuned. Contact me if you would like to be kept informed of our coming LED products.
This is an ideal set up for intravital microscopy on live animals. Such systems can also be used for the study of art objects and paintings. Industrial inspection of wafers (contaminants and excess photo-resist) can be identified easily with a fluorescence microscope. Special XY stages are available for large samples or XY movement of small animals etc.
LED MODULES MOUNTED ON A FLEXIBLE GOOSENECK ARE THE PERFECT SOLUTION FOR PHOTOMACROGRAPHY ILLUMINATION...THE LIGHT GETS INTO THE "NOOKS AND CRANNIES" AND IS EASILY INITIATED WITH A DSLR CAMERA AND RETRO-ZED. SHADOW-FREE ILLUMINATION OF 3D OBJECTS IS THE BEST LIGHTING SOLUTION...BUT SOMETIMES YOU WANT SHADOWS. A SERIOUS PHOTOMACROGRAPHER WILL HAVE BOTH RING ILLUMINATION AND POINT SOURCE LIGHTING (FLASH OR GOOSENECK TYPE) AT THEIR DISPOSAL. WE CAN TELL YOU MORE ABOUT FILTERS, POL FILTERS, COLLECTOR OPTICS AND SOFT LIGHT CREATION IF YOU ARE INTERESTED....JUST DROP US A NOTE OR SEND US YOUR SAMPLES FOR A PHOTO SESSION HERE AT OUR SHOP. WE THEN RETURN YOUR SAMPLES AND JPEG IMAGES BY EMAIL. YES, WITHOUT PROPER LIGHT FOR THE SAMPLE YOU WILL END UP WITH UNUSEABLE PHOTOS....WYSIWYG!
PHOTO LOWER RIGHT: www.lumecube.com
BUNTON SELLS THIS UNIT ALONG WITH A WIDE RANGE OF GOOSENECK CARRIERS...STATIC LED OR HIGH INTENSITY FLASH SYNCH FOR UNDER $100.00 (MADE IN AMERICA WITH 1/4-20 FEMALE FITTING, INTENSITY ADJUSTMENT AND WIRELESS OPERATION WITH RECHARGEABLE BATTERY VIA USB CABLE. COLOR FILTERS OPTIONS...DIFFUSOR INCLUDED ALONG WITH HOT SHOE MOUNT FOR YOUR DSLR/MILC. GREAT PRODUCT!
CHECK OUT: www.adaptalux.com THIS UK FIRM HAS A GREAT "MACRO FLASH" KIT THAT IS AN EXCELLENT CHOICE FOR CLOSE-UP AND PHOTOMACRO WORK. CHECK OUT THEIR YOUTUBE DEMO. A TWIN XENON FLASH HEAD KIT IS ABOUT $500.00. FLASH HEADS MAY BE USED IN CONJUNCTION WITH THE STATIC LED FLEXIBLE ARMS FOR MODELING AND COMPLEMENTARY COLORS FOR BACKGROUND ...PERFECT FOR PHOTOMACRO WORK, Z-STACKING AND STOP ACTION PHOTOMACROGRAPHY OF LIVE INSECTS. PURCHASE DIRECT FROM ADAPTALUX ON LINE. ORDER DIRECTLY ON-LINE FROM THEIR WEBSITE IN THE UK. MENTION BUNTON INSTRUMENT WHEN ORDERING FROM ADAPTALUX.
The photo (below LEFT #1) is the newest multi-LED unit we will be selling in 2023. This economical unit from China is an excellent price performance retrofit to replace mercury arc lamps on fluorescence microscopes. Available from Bunton for OEM customers as well as individual end-users. We have our own adapters for the various legacy Leitz microscopes as well as the current models from NOZL makes. Shown with Leitz Laborlux S upright. TRIPLE LED set up in one unit with remote control included to turn on/off as well as adjust intensity of each wavelength (365, 470 and 560nm ...VIOLET, BLUE and GREEN. Use your existing filter cubes or contact us if you need replacement filter sets (they do not last forever you know!). New hard coatings from CHROMA IN VERMONT provide excellent filtration for any current fluorochrome and we can reuse your filter cube to save you $300-800.00. New filter installation is included in the price of the filter set and dichroic. Optimize for DAPI, GFP/FITC and TRITC etc. Image shown is a MICROPLASTICS sample under 10x objective mag using a Leitz filter cube "B2". Mercury arc lamps all have hot spots and some difficulty in setting up for uniform field illumination. The knob on top of the LED housing allows adjustment of the collector lens for optimal illumination for all objective magnifications.
Detecton of microplastics and cyanbacteria (blue-green algae) in water samples. Clinical fluorescence microscopy for detection of various maladies. Research on living cells using GFP (green fluorescing protein)...and many more applications involving the famous Leitz Ploem illuminators and filter cubes. Yes, LED has pretty much replaced the mercury arc lamp these days.
3-LAMBDA model is under $3000.00 A 4-LAMBDA model is also available with added NIR excitation spike that sells for $3499.00.
CONTACT ME REGARDING LED UPGRADE FOR YOUR EXISTING NOZL MICROSCOPE. BESPOKE SYSTEMS ARE ALWAYS OF INTEREST AND WE HAVE THE EXPERTISE AND DESIGN/FABRICATION CAPABILITIES TO PROVIDE YOU WITH THE BEST DIGITAL MICROSCOPY SOLUTION TO MEET YOUR RESEARCH NEEDS AND BUDGET LIMITS. LED UPGRADES START AT UNDER $200.00.
ANOTHER USA-MADE LED WHITE LIGHT ILLUMINATOR IS NOW AVAILABLE FOR THOSE DOING INCIDENT LIGHT DARKFIELD, DIC OR POLARIZED LIGHT METHODS. ADAPTERS TO MOUNT THIS LED MODULE TO YOUR EXISTING MICROSCOPE....REPLACES 100W HALOGEN OR SMALL WATTAGE LAMPS THAT ARE INSUFFICIENT FOR SERIOUS PHOTOMICROSCOPY USING THESE METHODS......STANDARD INTENSITY MODEL IS ABOUT $550.00 WITH THE ADAPTER FOR YOUR MICROSCOPE INCLUDED.
A HIGH INTENSITY MODEL IS OFFERED FOR INCIDENT LIGHT INVESTIGATIONS INVOLVING OPAQUE SAMPLES BEING VIEWED IN DARKFIELD, POLARIZED LIGHT OR DIC. THESE MODELS ARE ABOUT $725.00. BOTH ARE AVAILABLE FOR USE ON OLDER NIKON, OLYMPUS AND ZEISS MICROSCOPES.
CONTACT US FOR A QUOTATION. WE ACCEPT USA UNIVERSITY AND GOVERNMENT PURCHASE ORDERS WITH NET 30 PAYMENT TERMS. INDIVIDUALS AND PRIVATE COMPANIES MAY USE PAYPAL OR WIRE TRANSFER BANK TO BANK. PRIVATE SECTOR CHECKS ACCEPTED WITH APPROPRIATE CLEAR TIME IN ADVANCE OF SHIPMENT.